in the field

Tag: ocean acidification

When I Stopped Chasing The Dream Job, I Discovered My Dream Career

Re-posting an article I wrote for National Girls Collaborative Project, because I’ve added fun links in this version! You can read the original article published on National Girls Collaborative Project Blog  (submitted by Greta Carlson on April 29, 2016 – 2:20 pm)

Contributed by Allison Lee 

Growing up I never had a clear picture of what I wanted to become. Inspecting insects, creating mud-pies and gazing at the stars were childhood past times and as I grew into a teenager I embraced traveling and learning more about nature. I didn’t immediately connect all of these interests with becoming a scientist. My parents were not scientists. No one in my family had degrees beyond a high school diploma.  I remember wondering if I even needed to go to college. I spent  years frustrated by my lack of vision; finding the dream job I felt contained all of my passions was a struggle.

By the time I turned 30, I had traveled to all seven continents as a Biologist studying a diversity of lifeforms from microscopic algae, to songbirds, parrots, mice, squirrels, big cats, and whales. Obviously I figured something out! Life is funny that way. A hunch paired with the right blend of curiosity, odd jobs, research-focused vacations, volunteer opportunities, frugal living, and luck delivered me to my dream career.

Finding my passion
In my high school senior yearbook I wrote that I wanted to be an astronaut. I thought astronauts traveled the most of anyone on Earth. Inspired, I researched the degrees earned by NASA’s astronauts and found an overwhelming proportion had studied science. I decided to major in biology and geology at the University of Washington. It wasn’t until sophomore year when I had the epiphany that biology was my passion.

The scope of biology is broad and I wanted to learn as many disciplines as possible. Senior year, I signed up for an internship in neuroscience at the Allen Institute for Brain Science and found a part-time lab job in the Immunology Department taking care of a mouse colony. I started learning molecular techniques and computer programs, using fancy pieces of equipment, and doing those things called PCRs and Southern Blots. I wasn’t entirely sure what was going on – but I was doing SCIENCE!

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Questioning my interests
After I graduated with my Bachelor of Science in Biology, I continued to work full time in the immunology lab. Two and a half years passed. Restlessness set in and I began to wonder if maybe I just didn’t like science anymore. I knew one next possible move was to go to graduate school, like many of my college peers, but I didn’t know what I wanted to commit to studying. I wasn’t inspired by my neurobiology or immunology experiences. I hated doing computer work. I felt stuck. So I did the thing you’re never supposed to do: I quit.

The next two months were spent feverishly searching job boards, institute websites, and email listservs. Because of my interest in many aspects of biology, I didn’t know where to focus my efforts. Regardless, I felt optimistic that my Dream Job was out there. A job posting for a field technician conducting songbird research caught my eye. I didn’t know much about birds, but I convinced the interviewer I could learn bird songs because I learned other languages easily and had been playing music for 15 years. It worked! Every morning I woke at 4:00 AM to get to the field site before the sun rose. I got paid to be outside tromping through the woods looking for birds. It was during these quiet twilight mornings, outside in nature, that I realized THIS was my dream job. I never wanted it to end.

But, as is the nature of seasonal fieldwork, it did. Five months later, I found myself once again unemployed. Turns out, seasonal work is the usual for field technicians. I knew that field biology wouldn’t be the most lucrative career choice, but I felt empowered to commit myself to studying the environment – whether I got paid for it or not.

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The sacrifices
I went on a quest to find as many wildlife jobs as I could. Later that year, I studied forest fire treatments on threatened squirrel populations; I studied the breeding behaviors of endangered parrots in Mexico. Each time, the pay was minimal to none, so I worked side-gigs as a professional baker and bartender to pay my bills. While I was happy to gallivant around the woods for science, working for free wasn’t exactly the dream job I had in mind. I was turning into a poor disgruntled vagabond.

In an effort to gain a sustainable salary, I left fieldwork and went back into the lab to begin a project at the Institute for Systems Biology (ISB) investigating the genetic responses of algae to climate change and ocean acidification. I knew nothing about marine systems but I had skills in the lab and stellar references. Part of me thought leaving the field was a mistake but as I gained exposure to the natural world within a molecular science framework, I saw the impact of a systems approach to research and I was hooked. I still missed going out into nature every day, but I kept an open mind and a positive attitude.

Over six years have passed since I went back into the lab and the job has given me more opportunities than I could have imagined. What started off as a lab job performing controlled experiments on algae has evolved into a field job sampling algae from the natural environment. This work has led me all over the Puget Sound area of Washington, as well as to Hawaii, and even as far as Antarctica!

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The best of both worlds
As a field scientist working indoors, I still daydreamed about the rainforests. On the weekends, I’d flee to the mountains. With a bit of money saved up, I decided to satisfy my restless soul. I used my vacations to help conduct research on voluntourism projects, like Earthwatch. From aboard a restored rubber boom era steamship, I spent three weeks studying biodiversity in the Amazon jungle. I realized I could save my money, use my vacation time to get out in the field, and still have a paying job in science. It was the best of both worlds!

The plan is working. I’ve volunteered on projects studying macaws with Tambopata Research Center in Peru, biodiversity in the Amazon, big cats in Africa, and whales in Mexico. I love volunteering so much I do it at home too, giving time to local conservation organizations like Conservation Northwest. We work to mitigate human wildlife conflicts in the migration corridors of the Cascades. I also get kids outdoors with the Sierra Club, and spend time with kids through Ocean Inquiry Project, the Woodland Park Zoo, and Pacific Science Center. I speak about careers in environmental science with students to convey my passion for nature and inspire another generation to care about protecting the biodiversity on Earth.

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What’s in a dream?
I’ve come to believe the dream job doesn’t exist for me, and I don’t think it’s beneficial to maintain that perspective. I have too many passions – too much wonder for the potential of science in the world. I find fulfillment in contributing to important work for the environment and interacting with innovative collaborative thinkers. I benefit from an extremely flexible schedule, live in the city, and travel to amazing places through work. And I’ve been lucky to have many other amazing experiences volunteering with local and international conservation organizations. I am continually seeking as many opportunities as I can find, but this time things are different; I’m no longer looking for the Dream Job, I am creating the Dream Career.

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Allison Lee is a Seattle native who loves coffee, talking to people, and learning new things. When she’s not doing science you can catch her ultra-running, cycling, hiking in nature, traveling, and burning the candle at both ends. At age 32, she has finally realized the differences between working a job and building a career. This summer she will begin a Masters degree in Marine Biodiversity and Conservation at Scripps Institution of Oceanography in San Diego California with the hopes of following another curiosity: the social, economic and policy side of marine science. You can follow her and see examples of other inspirational women working in the field at Woman Scientist on Instagram, Facebook, and the Web.

**UPDATE** (April 2018): Allison graduated with her Master’s degree June 2017 and shortly after received funding from the National Science Foundation to support the Capstone project she created with Dr. Maria Vernet (watch the video here). Allison is now in the Biological Oceanography PhD program with Dr. Vernet at Scripps Institution of Oceanography leading the FjordPhyto citizen science project with tourists in Antarctica and the Arctic. She also works part time as a Senior Research Associate at Synthetic Genomics (Target CW) to advance breakthroughs in algal biofuel research.

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The Invisible Forest Hiding Under Water

We’ve all gone swimming in the lakes and in the seas. We’ve all choked on that accidental gulp of water while swimming carefree.

But have we all looked under a microscope to see what these waters hold?

What you just saw were three plant-like organisms called diatoms, and the moving thing: a small larval crustacean called a nauplius. Makes you think differently about that next gulp of water you’ll take, doesn’t it?

I had previously never given much thought to the invisible marine world until I was employed to work on a project researching the effects of climate change on micro-algae, particularly the diatom Thalassiosira pseudonana. 

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What a beaut!

Who is this Thalassio-whatchamacallit and why should I care? Let me ask you a question:

Where does the earth’s oxygen come from? Humans, as the terrestrial beings we are, will easily shout out, “The trees!” But did you know the trees are only responsible for producing about 30% of that oxygen? The algae and phytoplankton living in the water are responsible for the other 50-70%! That is more than half!! In addition, these amazing silicified (glass) microorganisms make up the base of the food web and are responsible for 40% of the ocean’s primary productivity. What an incredible hidden forest.

I will never look at the ocean in the same way.

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Check out their distribution around the world 

Phytoplankton can be identified by NASA satellites through their chlorophyll

Phytoplankton as Art 

I learned of a man named Klaus Kemp, nicknamed The Diatomist, who also doesn’t look at the ocean in the same way. He sees magnificent beauty in the Invisible Forest and  has spent his life mastering the Victorian art of creating kaleidoscope-like arrangements of diatoms.

I don’t think I could have the patience or attention span to do such painstaking technical arrangements under a microscope but don’t get me wrong, I do love a good microscope session attempting to photograph some of the world’s smallest organisms.

Capturing Invisible Things

The best way to collect a large abundance of phytoplankton is to do a tow with a net that has a good funnel-shaped collection cylinder called a cod end. Some nets are huge and require winches to lower into the water. Others are more manageable and can be done by hand, towed behind a row boat.

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Phytoplankton can range in size from 2 – 1000 microns.  What does that even look like? If you run sea water through a mesh filter that had a 333 micron weave, you could see Coscinodiscus wailesii by eye; It looks like tiny grains of sand. Does that help the visual reference at all?

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Unannounced Changes

For three weeks, from March 17, 2015 until April 3, 2015,  I worked up in the Pacific Northwest’s San Juan Islands at the University of Washington Friday Harbor Labs. My boss and Co. were running ocean acidification experiments on our lab strain diatom, Thalassiosira pseudonana, but we wanted to also collect the wild plankton living in the harbor.  Weekly we would take the row boat out to do a tow. I attempted to photograph what I saw using only an iPhone through the microscope:

Week 1: We saw many different types of diatoms: Thalassiosira, Melosira, Coscinodiscus, Chaetoceros, Ditylum, Thalassionema, Skeletonema, Cylindrotheca, & Corethron

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Week 2: The diatoms had mostly been replaced with dinoflagellates (Protoperidinium, Dinophysis) and zooplankton, such as the crustacean copepods.

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Week 3: The waters were teeming with copepods and tiny little jellies: Cnidaria and Ctenophora.

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Week 4: Ctenophores had disappeared and a diverse collection of diatoms and dinoflagellates floated amongst a gazillion copepods. (Diatoms: Thalassiosira, Coscinodiscus, Ditylum, Chaetoceros, Odeontella, Thalassionema, Skeletonema, Bacillaria, Cylindrotheca, Triceratium; Dinoflagellates: Ceratium, Protoperidinium.)

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While I would not spend hours arranging any of these into beautiful mosaics, I would spend hours trying to photograph them as they are.

Lab Diatoms Go Wild  

What experiments are we doing with these critters at the Friday Harbor Labs that we can’t do back at the Institute’s labs in Seattle? Mesocosm Experiments. Mesocosms are a bridge between controlled laboratory experiments (which use artificially made sea water) and the more variable and uncontrolled field environment (which use natural sea water coming from the harbor).  In 2011 the University of Washington invested money from grants, and private donors to build an amazing Ocean Acidification Environmental Lab with pimped out coolers that have the ability to monitor and control experimental conditions.

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The sea water in this area is naturally more acidic due to freshwater river run-off, coastal upwelling and anthropogenic practices.  The pH of the average ocean is said to be around 8.1 reflecting the average atmospheric CO2 levels at 400ppm. The pH of the waters at Friday Harbor is 7.8 with a recorded dissolved carbon dioxide level of 650ppm. We are using this water with all its organic goodies to test how our lab strain of Diatom will respond genetically to  future predicted decreases in pH (due to increases in dissolved carbon dioxide).

Thalassiosira pseudonana is an algae that creates a silica glass shell. Because of this, they do not have the same need for calcium as corals, oysters and other shellfish do. This means when carbon dioxide dissolves into the sea water, snatching up available calcium, this particular organism doesn’t seem to suffer. In fact, with increase CO2 this diatom is able to relax its carbon concentrating mechanisms, passively allowing CO2 to dissolve through its glass frustule. In the process of photosynthesis, the ability for the plant to sequester much of the increased CO2,  is referred to as CO2 fertilization. To read the final publication on what we found in the laboratory experiments click the paper below:

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Breathe

Next time you take a deep breath, thank these under-appreciated organisms which make up the Invisible Forest and give us the oxygen we need to survive.

Want more? 

To see more photos of the entire spring trip to Friday Harbor, click here:

To see photos from a previous fall trip taken in September 2014, click here.

Photo Credits:

Hsiao-Ching Chou: Allison wearing gloves holding net; Gloved hand holding cod-end; Mesocosms.

Spiro Jamie: Allison holding smallest net.

Allison Lee: All other photos except NASA chlorophyll distribution image and TEM image of Thalassiosira pseudonana.

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